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| 科研成果 |
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| 论文题目： |
Determination of Cell Membrane Capacitance and Conductance via Optically Induced Electrokinetics |
| 第一作者： |
Liang WF(梁文峰);Zhao YL(赵玉良);Liu LQ(刘连庆);Wang YC(王越超);Li WR(李文荣);Li GB(李国宾) |
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| 发表刊物： |
BIOPHYSICAL JOURNAL |
| 发表年度： |
2017 |
| 卷,期,页： |
113,7,1531-1539 |
| 论文出处： |
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| 论文摘要： |
Cell membrane capacitance and conductance are key pieces of intrinsic information correlated with the cellular dielectric parameters and morphology of the plasma membrane; these parameters have been used as electrophysiological biomarkers to characterize cellular phenotype and state, and they have many associated clinical applications. Here, we present our work on the non-invasive determination of cell membrane capacitance and conductance by an optically activated microfluidics chip. The model for determining the cell membrane capacitance and conductance was established by a single layer of the shellcore polarization model. Three-dimensional finite-element analyses of the positive and negative optically induced dielectrophoresis forces generated by the projected light arrays of spots were performed, thus providing a theoretical validation of the feasibility of this approach. Then, the crossover frequency spectra for four typical types of cells (Raji cells, MCF-7 cells, HEK293 cells, and K562 cells) were experimentally investigated by using a micro-vision based motion-tracking technique. The different responses of these cells to the positive and negative ODEP forces were studied under four different liquid conductivities by automatic observation and tracking of the cellular trajectory and texture during the cells' translation. The cell membrane capacitance and conductance were determined from the curve-fitted spectra, which were 11.1 +/- 0.9 mF/m(2) and 782 +/- 32 S/m(2), respectively, for Raji cells, 11.5 +/- 0.8 mF/m(2) and 114 +/- 28 S/m(2) for MCF-7 cells, 9.0 +/- 0.9 mF/m(2) and 187 +/- 22 S/m(2) for HEK293 cells, and 10.2 +/- 0.7 mF/m(2) and 879 +/- 24 S/m(2) for K562 cells. Furthermore, as an application of this technique, the membrane capacitances of MCF-7 cells treated with four different concentrations of drugs were acquired. This technique introduces a determination of cell membrane capacitance and conductance that yields statistically significant data while allowing information from individual cells to be obtained in a non-invasive manner. |
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